Love as a force that overrides reason
The potion makes the point literally, but even before it appears, the lovers act irrationally. Hermia defies her father and the law. Helena chases a man who rejects her. Shakespeare shows love as something that bypasses logic, and the play treats that as funny rather than tragic.
Order versus chaos
Athens represents rigid social order: laws, fathers, dukes. The forest represents chaos: shifting identities, reversed loyalties, magic. The play moves characters from one world into the other and back again, and they return changed. The tension between the two worlds drives every plot line.
Appearance versus reality
Characters constantly misread what they see. The lovers cannot tell genuine feeling from enchanted feeling. Titania sees beauty in Bottom's donkey head. Bottom cannot tell his dream from reality. The play keeps asking whether what we perceive is ever actually true.
Power and control in relationships
Egeus controls Hermia. Oberon controls Puck and tries to control Titania. Demetrius initially controls Helena through rejection. The play distributes power unevenly and then uses magic and comedy to redistribute it, though not always fairly.
Art, performance, and illusion
The craftsmen's subplot puts a play inside the play. Their bad performance of Pyramus and Thisbe forces the audience to think about what theater does and how much we agree to believe. Puck's final speech extends that question to the whole play.